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Extraction and preconcentration of tylosin from milk samples through functionalized TiO 2 nanoparticles reinforced with a hollow fiber membrane as a novel solid/liquid‐phase microextraction technique
Author(s) -
Sehati Negar,
Dalali Nasser,
Soltanpour Shahla,
Seyed Dorraji Mir Saeed
Publication year - 2014
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201400393
Subject(s) - solid phase microextraction , extraction (chemistry) , tylosin , materials science , chromatography , detection limit , fiber , enrichment factor , sample preparation , solid phase extraction , membrane , hollow fiber membrane , chemistry , composite material , mass spectrometry , gas chromatography–mass spectrometry , biochemistry , antibiotics
The aim of this study was to introduce a novel, simple, and highly sensitive preparation method for determination of tylosin in different milk samples. In the so‐called functionalized TiO 2 hollow fiber solid/liquid‐phase microextraction method, the acceptor phase is functionalized TiO 2 nanoparticles that are dispersed in the organic solvent and held in the pores and lumen of a porous polypropylene hollow fiber membrane. An effective functionalization of TiO 2 nanoparticles has been done in the presence of aqueous H 2 O 2 and a mild acidic ambient under UV irradiation. This novel extraction method showed excellent extraction efficiency and a high enrichment factor (540.2) in comparison with conventional hollow fiber liquid‐phase microextraction. All the experiments were monitored at λ max = 284 nm using a simple double beam UV‐visible spectrophotometer. A Taguchi orthogonal array experimental design with an OA 16 (4 5 ) matrix was employed to optimize the factors affecting the efficiency of hollow fiber solid/liquid‐phase microextraction such as pH, stirring rate, salt addition, extraction time, and the volume of donor phase. This developed method was successfully applied for the separation and determination of tylosin in milk samples with a linear concentration range of 0.51–7000 μg/L ( r 2 = 0.991) and 0.21 μg/L as the limit of detection.