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Isolation and purification of diastereoisomeric flavonolignans from silymarin by binary‐column recycling preparative high‐performance liquid chromatography
Author(s) -
Zhao Weiquan,
Yang Guang,
Zhong Fanyi,
Yang Nan,
Zhao Xin,
Qi Yunpeng,
Fan Guorong
Publication year - 2014
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201400270
Subject(s) - chromatography , chemistry , high performance liquid chromatography , isolation (microbiology) , biology , microbiology and biotechnology
Silymarin extracted from Silybum marianum (L.) Gaertn consists of a large number of flavonolignans, of which diastereoisomeric flavonolignans including silybin A and silybin B, and isosilybin A and isosilybin B are the main bioactive components, whose preparation from the crude extracts is still a difficult task. In this work, binary‐column recycling preparative high‐performance liquid chromatography systems without sample loop trapping, where two columns were switched alternately via one or two six‐port switching valves, were established and successfully applied to the isolation and purification of the four diastereoisomeric flavonolignans from silymarin. The proposed system showed significant advantages over conventional preparative high‐performance liquid chromatography with a single column in increasing efficiency and reducing the cost. To obtain the same amounts of products, the proposed system spends only one tenth of the time that the conventional system spends, and needs only one eleventh of the solvent that the conventional system consumes. Using the proposed system, the four diastereoisomers were successfully isolated from silymarin with purities over 98%.