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Extraction of aflatoxins from food samples using graphene‐based magnetic nanosorbents followed by high‐performance liquid chromatography: A simple solution to overcome the problems of immunoaffinity columns
Author(s) -
Es'haghi Zarrin,
Reza Beheshti Hamed,
Feizy Javad
Publication year - 2014
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201400260
Subject(s) - chromatography , simple (philosophy) , chemistry , extraction (chemistry) , high performance liquid chromatography , graphene , aflatoxin , materials science , nanotechnology , food science , philosophy , epistemology
In this research, magnetic graphene nanoparticles were prepared and used as adsorbents for preconcentrating the aflatoxins in rice, wheat, and sesame samples. For this purpose, graphene was synthesized by Hummer's method. Magnetically modified graphene formed by the deposition of magnetite (Fe 3 O 4 ) on graphene was used for the separation of aflatoxins B 1 , B 2 , G 1 , and G 2 from the samples. The extractants were subsequently analyzed with high‐performance liquid chromatography and fluorescence detection. Parameters affecting the efficiency of the method were thoroughly investigated. The measurements were done under the optimized conditions. For aflatoxins B 1 , B 2 , G 1 , and G 2 , limits of detection were 0.025, 0.05, 0.05, and 0.075 ng/g and limits of quantification were 0.083, 0.16, 0.16, and 0.23 ng/g, respectively. Accuracy was examined by the determination of the relative recovery of the aflatoxins. The relative recovery of aflatoxins B 1 , B 2 , G 1 , and G 2 were quite satisfactory (between 64.38 and 122.21% for food samples). Relative standard deviations for within laboratory repeatability ( n = 6) were in the range from 1.3 to 3.2. The application of this sorbent for the separation and concentration of the mentioned aflatoxins from food samples was examined.