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Testing the capability of a polynomial‐modified gaussian model in the description and simulation of chromatographic peaks of amlodipine and its impurity in ion‐interaction chromatography
Author(s) -
Čolović Jelena,
Vemić Ana,
Kostić Nađa,
Malenović Anđelija,
Medenica Mirjana
Publication year - 2014
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201400206
Subject(s) - chromatography , impurity , amlodipine , chemistry , polynomial , gaussian , materials science , mathematics , organic chemistry , computational chemistry , mathematical analysis , medicine , blood pressure , radiology
In this paper, the capability of a polynomial‐modified G aussian model to relate the peak shape of basic analytes, amlodipine, and its impurity A, with the change of chromatographic conditions was tested. For the accurate simulation of real chromatographic peaks the authors proposed the three‐step procedure based on indirect modeling of peak width at 10% of peak height ( W 0.1 ), individual values of left‐half width ( A ) and right‐half width ( B ), number of theoretical plates ( N ), and tailing factor (Tf). The values of retention factors corresponding to the peak beginning ( k B ), peak apex ( k A ), peak ending ( k E ), and peak heights ( H 0 ) of the analytes were directly modeled. Then, the investigated experimental domain was divided to acquire a grid of appropriate density, which allowed the subsequent calculation of W 0.1 , A , B , N , and Tf. On the basis of the predicted results for Tf and N , as well as the defined criteria for the simulation the following conditions were selected: 33% acetonitrile/67% aqueous phase (55 mM perchloric acid, pH 2.2) at 40°C column temperature. Perfect agreement between predicted and experimental values was obtained confirming the ability of polynomial modified G aussian model and three‐step procedure to successfully simulate the real chromatograms in ion‐interaction chromatography.