Premium
Investigating retention characteristics of a mixed‐mode stationary phase and the enhancement of monolith selectivity for high‐performance liquid chromatography
Author(s) -
Bassanese Danielle N.,
Soliven Arianne,
Stevenson Paul G.,
Dennis Gary R.,
Barnett Neil W.,
Shalliker Ross A.,
Conlan Xavier A.
Publication year - 2014
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201400201
Subject(s) - monolith , selectivity , chemistry , silane , acetonitrile , alkyl , chromatography , high performance liquid chromatography , solvent , methanol , organic chemistry , catalysis
The synthesis and chromatographic behavior of an analytical size mixed‐mode bonded silica monolith was investigated. The monolith was functionalized by an in situ modification process of a bare silica rod with chloro(3‐cyanopropyl)dimethyl silane and chlorodimethyl propyl phenyl silane solutions. These ligands were selected in order to combine both resonance and nonresonance π‐type bonding within a single separation environment. Selectivity studies were undertaken using n ‐alkyl benzenes and polycyclic aromatic hydrocarbons in aqueous methanol and acetonitrile mobile phases to assess the methylene and aromatic selectivities of the column. The results fit with the linear solvent strength theory suggesting excellent selectivity of the column was achieved. Comparison studies were performed on monolithic columns that were functionalized separately with cyano and phenyl ligands, suggesting highly conjugated molecules were able to successfully exploit both of the π‐type selectivities afforded by the two different ligands on the mixed‐mode column.