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Poly(glycidyl methacrylate‐ co ‐ N ‐methylolacrylamide‐ co ‐ethylene dimethacrylate) monolith coupled to high‐performance liquid chromatography for the determination of adenosine phosphates in royal jelly
Author(s) -
Liu Dan,
Zhang Tianbin,
Cheng Yechun,
Jia Qiong
Publication year - 2014
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201400187
Subject(s) - glycidyl methacrylate , monolith , monolithic hplc column , polymerization , methacrylate , ethylene glycol dimethacrylate , monomer , chromatography , chemistry , polymer , high performance liquid chromatography , polymer chemistry , materials science , chemical engineering , methacrylic acid , organic chemistry , catalysis , engineering
A polymer monolith microextraction method coupled with high‐performance liquid chromatography was developed for the determination of adenosine triphosphate, adenosine diphosphate, and adenosine monophosphate. The monolithic column was synthesized inside fused‐silica capillaries using thermal initiation free‐radical polymerization with glycidyl methacrylate as the monomer, ethylene dimethacrylate as the cross‐linker, cyclohexanol, and 1‐dodecanol as the porogen. N ‐Methylolacrylamide, an important hydrophilic monomer, was incorporated into the polymerization mixture to enhance the hydrophilicity of the poly(glycidyl methacrylate‐ co ‐ethylene dimethacrylate) column. The obtained poly(glycidyl methacrylate‐ co ‐ N ‐methylolacrylamide‐ co ‐ethylene dimethacrylate) monolith was characterized by scanning electron microscopy, Fourier‐transform infrared spectra, and X‐ray photoelectron spectroscopy. Optimum conditions for the preconcentration and separation of the target adenosines were also investigated. Under the optimum conditions, we obtained acceptable linearities, low limits of detection, and good relative standard deviations. The developed polymer monolith microextraction with high‐performance liquid chromatography method exhibited a good performance with recovery values in the range of 76.9−104.7% when applied to the determination of the adenosines in five royal jelly samples.