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Integrating qualitative and quantitative characterization of traditional C hinese medicine injection by high‐performance liquid chromatography with diode array detection and tandem mass spectrometry
Author(s) -
Xie Yuanyuan,
Xiao Xue,
Luo Juanmin,
Fu Chan,
Wang Qiaowei,
Wang Yiming,
Liang Qionglin,
Luo Guoan
Publication year - 2014
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201400129
Subject(s) - chromatography , chemistry , mass spectrometry , tandem mass spectrometry , rosmarinic acid , high performance liquid chromatography , chromatography detector , electrospray , antioxidant , organic chemistry
The present study aims to describe and exemplify an integrated strategy of the combination of qualitative and quantitative characterization of a multicomponent mixture for the quality control of traditional C hinese medicine injections with the example of D anhong injection ( DHI ). The standardized chemical profile of DHI has been established based on liquid chromatography with diode array detection. High‐performance liquid chromatography coupled with time‐of‐flight mass spectrometry and high‐performance liquid chromatography with electrospray multistage tandem ion‐trap mass spectrometry have been developed to identify the major constituents in DHI . The structures of 26 compounds including nucleotides, phenolic acids, and flavonoid glycosides were identified or tentatively characterized. Meanwhile, the simultaneous determination of seven marker constituents, including uridine, adenosine, danshensu, protocatechuic aldehyde, p ‐coumaric acid, rosmarinic acid, and salvianolic acid B , in DHI was performed by multiwavelength detection based on high‐performance liquid chromatography with diode array detection. The integrated qualitative and quantitative characterization strategy provided an effective and reliable pattern for the comprehensive and systematic characterization of the complex traditional Chinese medicine system.