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Thermoelectric‐based temperature‐controlling system for in‐tube solid‐phase microextraction
Author(s) -
Yang Yang,
RodriguezLafuente Angel,
Pawliszyn Janusz
Publication year - 2014
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201400041
Subject(s) - thermoelectric effect , solid phase microextraction , materials science , extraction (chemistry) , detection limit , analytical chemistry (journal) , calibration curve , desorption , chromatography , solid phase extraction , atmospheric temperature range , chemistry , mass spectrometry , gas chromatography–mass spectrometry , adsorption , thermodynamics , physics , organic chemistry
A temperature‐controlling device for in‐tube solid‐phase microextraction was developed based on thermoelectric cooling and heating. This device can control the temperature of the capillary column from 0 to 100°C by applying a voltage to a Peltier cooler or stainless steel tube. The extraction temperatures for angiotensin I, propranolol, and ranitidine were optimized. In all cases, setting the temperature to 10°C for extraction achieved the best extraction efficiency. Desorption showed minimum peak broadening at 70°C, contributing to better chromatographic performance. Propranolol was selected as a model compound to compare the performance of temperature‐controlled in‐tube solid‐phase microextraction at optimized conditions. Calibration curves exhibited good linearity ( R 2 > 0.999) over the studied range, and the limit of detection and limit of quantification were about three times lower than those obtained at standard conditions (30°C extraction and desorption).