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Study of the retention behavior of small polar molecules on different types of stationary phases used in hydrophilic interaction liquid chromatography
Author(s) -
Vlčková Hana,
Ježková Kateřina,
Štětková Kateřina,
Tomšíková Helena,
Solich Petr,
Nováková Lucie
Publication year - 2014
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201400020
Subject(s) - chemistry , selectivity , chromatography , acetonitrile , phase (matter) , hydrophilic interaction chromatography , high performance liquid chromatography , stationary phase , reversed phase chromatography , analytical chemistry (journal) , analyte , polar , organic chemistry , physics , astronomy , catalysis
The retention behavior of a large group of analytes (35) with varied properties (p K a and log P ) was studied on eight hydrophilic interaction LC columns with different surfaces, stationary phase chemistries, and types of particles. The acetonitrile content (5–95%), buffer concentration (0.5–200 mM), and pH of the mobile phase (3.8 and 6.8) were evaluated for their effects on the retention behavior. The type of stationary phase had a significant impact on the selectivity and retention time of the tested analytes. Completely different selectivity was observed on the aminopropyl stationary phase. In this study, the influence of the buffer concentration was similar for all tested columns, except for the aminopropyl stationary phase. Increasing the buffer concentration led to decreased retention times for the basic compounds and increased retention times for the acidic compounds, while the inverse behavior was observed on the aminopropyl stationary phase. The selectivity of the individual stationary phases was evaluated at pH 3.8 and 6.8. Much lower selectivity differences between the stationary phases were observed at pH 6.8 than pH 3.8. Bare silica stationary phases were used in the comparison of the particles (fused‐core and fully porous particles of 3 and 1.7 μm) and the columns provided by different manufacturers.