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Molecularly imprinted microspheres for the anticancer drug aminoglutethimide: S ynthesis, characterization, and solid‐phase extraction applications in human urine samples
Author(s) -
Lai JiaPing,
Chen Fang,
Sun Hui,
Fan Li,
Liu GuiLing
Publication year - 2014
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201400008
Subject(s) - chromatography , solid phase extraction , molecularly imprinted polymer , chemistry , suspension polymerization , extraction (chemistry) , molecular imprinting , urine , high performance liquid chromatography , polymerization , polymer , selectivity , organic chemistry , biochemistry , catalysis
Molecularly imprinted microspheres ( MIM s) for the anticancer drug aminoglutethimide ( AG ) were synthesized by aqueous suspension polymerization. The expected size and diameter of MIM s are controlled easily by changing one of the surfactant types, ratio of organic‐to‐water phase or stirring rate during polymerization. The obtained MIM s exhibit specific affinity toward AG with imprinting factor of 3.11 evaluated with a chromatographic model. The resultant MIM s were used as the SPE materials for the extraction of AG from human urine. A molecularly imprinted SPE ( MISPE ) method coupled with HPLC has been developed for the extraction and detection of AG in urine. Our results showed that most impurities from urine can be removed effectively after a washing step and the AG has been enriched effectively after MISPE operation with the recovery of >90% ( n = 3). The developed MISPE – HPLC method could be used for enrichment and detection of AG in human urine.