Premium
Chemical differentiation of volatile compounds in crude and processed A tractylodis M acrocephalae R hizoma by using comprehensive two‐dimensional gas chromatography with time‐of‐flight mass spectrometry combined with multivariate data analysis
Author(s) -
Cao Gang,
Cai Hao,
Jiang Jianping,
Yao Linjiang,
Tu Sicong,
Wang Lin,
Ma Xiaoqing,
Cai Baochang
Publication year - 2014
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201301376
Subject(s) - chemistry , principal component analysis , gas chromatography–mass spectrometry , mass spectrometry , chromatography , multivariate analysis , chemical constituents , gas chromatography , qualitative analysis , mathematics , qualitative research , social science , sociology , statistics
In the present study, comprehensive 2 D GC — TOF‐MS combined with multivariate data analysis was applied to analyze the differences of the volatile components in crude and processed A tractylodis M acrocephalae R hizoma ( AMR ) samples. As a result, 26 compounds that were found in crude AMR samples disappeared in processed AMR samples, and 19 compounds were newly generated and identified in AMR after processing with wheat bran. Meanwhile, principal component analysis demonstrated that there were significant chemical differences between crude and processed AMR samples, and processing procedure caused obvious quantitative and qualitative changes of volatile components in AMR . The established method could be used to explain the chemical differentiation between crude and processed AMR , and to further understand the processing mechanism of herbal medicines.