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Novel devices for solvent delivery and temperature programming designed for capillary liquid chromatography
Author(s) -
Marins Coutinho Lincoln Figueira,
Domingues Nazario Carlos Eduardo,
Monteiro Alessandra Maffei,
Lanças Fernando Mauro
Publication year - 2014
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201301324
Subject(s) - chromatography , syringe driver , volumetric flow rate , isothermal process , solvent , chemistry , capillary action , temperature control , process engineering , syringe , materials science , control engineering , psychology , physics , composite material , quantum mechanics , psychiatry , engineering , thermodynamics , organic chemistry
Analyses in chromatographic systems able to save mobile and stationary phases without reducing efficiency and resolution are of current interest. These advantages regarding savings have challenged us to develop a system dedicated to miniaturized liquid chromatography. This paper reports on the development of a high‐pressure syringe‐type pump, an oven able to perform isothermal and temperature programming and a software program to control these chromatographic devices. The experimental results show that the miniaturized system can generate reproducible and accurate temperature and flow rate. The system was applied to the separation of statins and tetracylines and showed excellent performance.