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Development and evaluation of gas and liquid chromatographic methods for the analysis of fatty amines
Author(s) -
Breitbach Zachary S.,
Weatherly Choyce A.,
Woods Ross M.,
Xu Chengdong,
Vale Glenda,
Berthod Alain,
Armstrong Daniel W.
Publication year - 2014
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201301265
Subject(s) - chemistry , chromatography , acetonitrile , ionic liquid , selectivity , gas chromatography , amine gas treating , alkyl , flame ionization detector , analytical chemistry (journal) , organic chemistry , catalysis
In contrast to the plethora of publications on the separation of fatty acids, analogous studies involving fatty amines are scarce. A recently introduced ionic‐liquid‐based capillary column for GC was used to separate trifluoroacetylated fatty amines focusing on the analysis of a commercial sample. Using the ionic liquid column (isothermal mode at 200°C) it was possible to separate linear primary fatty amines from C 12 to C 22 chain length in less 25 min with MS identification. The log of the amine retention factors are linearly related to the alkyl chain length with a methylene selectivity of 0.117 kcal/mol for the saturated amines and 0.128 kcal/mol for the mono‐unsaturated amines. The sp 2 selectivity for unsaturated fatty amines also could be calculated as 0.107 kcal/mol for the ionic liquid column. The commercial sample was quantified by GC with flame ionization detection ( FID ). An LC method also was developed with a reversed phase gradient separation using acetonitrile/formate buffer mobile phases and ESI ‐ MS detection. Native amines could be detected and identified by their single ion monitoring chromatograms even when partial coelution was observed. The analysis of the commercial sample returned results coherent with those obtained by GC – FID and with the manufacturer's data.