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Preconcentration based on paramagnetic microparticles for the separation of sarcosine using hydrophilic interaction liquid chromatography coupled with coulometric detection
Author(s) -
Zitka Ondrej,
Heger Zbynek,
Kominkova Marketa,
Skalickova Sylvie,
Krizkova Sona,
Adam Vojtech,
Kizek Rene
Publication year - 2014
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201301188
Subject(s) - chromatography , chemistry , sarcosine , coulometry , acetonitrile , analytical chemistry (journal) , electrochemistry , electrode , glycine , biochemistry , amino acid
Sarcosine has been identified as a potential prostate cancer marker. To provide determination of this compound, a number of methods are developing. In this study, we optimized a method for its separation by hydrophilic interaction LC with electrochemical detection ( ED ). Due to the fact that mobile phases commonly used for this type of separation altered the LOD s measured by electrochemical detectors, we applied postcolumn dosing of buffer suitable for ED . The optimized conditions were mobile phase A acetonitrile, mobile phase B water in the ratio A / B 70:30, with postcolumn addition of mobile phase C (200 mM phosphate buffer pH 9). The optimal mixing ratio was A + B / C 1:1 with a flow rate of 0.80 mL/min (0.40 + 0.40 mL/min) and detection potential of 1000 mV. Due to the optimization of the parameters for effective separation, which had to meet the optimal parameters of ED , we reached a good resolution for separation also with a good LOD (100 nM). In addition, we successfully carried out sarcosine analysis bound on our modified paramagnetic microparticles with the ability to preconcentrate sarcosine isolated from artificial urine.