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Selective extraction of clonazepam from human plasma and urine samples by molecularly imprinted polymeric beads
Author(s) -
Panahi Homayon Ahmad,
Mehramizi Ali,
Ghassemi Somayeh,
Moniri Elham
Publication year - 2014
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201301144
Subject(s) - molecularly imprinted polymer , sorbent , chemistry , chromatography , solid phase extraction , clonazepam , precipitation polymerization , suspension polymerization , extraction (chemistry) , polymer , thermogravimetric analysis , sorption , fourier transform infrared spectroscopy , polymerization , nuclear chemistry , radical polymerization , adsorption , chemical engineering , organic chemistry , selectivity , catalysis , psychology , psychiatry , engineering
A molecularly imprinted polymer ( MIP ) based on free‐radical polymerization was prepared with 1‐( N , N ‐biscarboxymethyl)amino‐3‐allylglycerol and N , N ‐dimethylacrylamide as functional monomers, N , N ‐methylene diacrylamide as the cross‐linker, copper ion‐clonazepam as the template and 2,2‐azobis(2‐methylbutyronitrile) as the initiator. The imprinted polymer was characterized by F ourier transform infrared spectroscopy, elemental analysis, thermogravimetric analysis, and SEM . The MIP of agglomerated microparticles with multipores was used for SPE . The imprinted polymer sorbent was selective for clonazepam. The optimum pH and sorption capacity were 5 and 0.18 mg/g at 20°C, respectively. The profile of the drug uptake by the sorbent reflects good accessibility of the active sites in the imprinted polymer sorbent. The MIP ‐ SPE was the most feasible technique for the extraction of clonazepam with a high recovery from human plasma and urine samples.