Selective extraction of clonazepam from human plasma and urine samples by molecularly imprinted polymeric beads

A molecularly imprinted polymer ( MIP ) based on free‐radical polymerization was prepared with 1‐( N , N ‐biscarboxymethyl)amino‐3‐allylglycerol and N , N ‐dimethylacrylamide as functional monomers, N , N ‐methylene diacrylamide as the cross‐linker, copper ion‐clonazepam as the template and 2,2‐azobis(2‐methylbutyronitrile) as the initiator. The imprinted polymer was characterized by F ourier transform infrared spectroscopy, elemental analysis, thermogravimetric analysis, and SEM . The MIP of agglomerated microparticles with multipores was used for SPE . The imprinted polymer sorbent was selective for clonazepam. The optimum pH and sorption capacity were 5 and 0.18 mg/g at 20°C, respectively. The profile of the drug uptake by the sorbent reflects good accessibility of the active sites in the imprinted polymer sorbent. The MIP ‐ SPE was the most feasible technique for the extraction of clonazepam with a high recovery from human plasma and urine samples.