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Robustness of a method based on the serial coupling of reversed‐phase and zwitterionic hydrophilic interaction LC – MS for the analysis of phenols
Author(s) -
Greco Giorgia,
Grosse Sylvia,
Letzel Thomas
Publication year - 2014
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201301112
Subject(s) - phenols , wine , chemistry , phenol , robustness (evolution) , chromatography , polar , mass spectrometry , stationary phase , analytical chemistry (journal) , organic chemistry , biochemistry , physics , food science , astronomy , gene
We have recently proved that the serial coupling of RP and zwitterionic hydrophilic interaction LC with mass spectrometric detection is a versatile and reliable technique to simultaneously separate polar and apolar compounds in complex samples, for example, phenols in wine. In order to evaluate the system suitability for long‐term usage, the robustness of a method based on the serial coupling of RP and zwitterionic hydrophilic interaction LC was evaluated after one year of analyses comprising >1100 injections. The retention time and peak shape of phenol standards and phenols in a red wine were compared to the values previously published. Phenol retention times were shifted <30 s. However, the peak widths were increased, partially due to the deterioration of the stationary phases. Nevertheless, the method was still highly reliable for the analysis of phenols in wine.