Premium
Determination of naproxen in human plasma by GC–MS
Author(s) -
Yilmaz Bilal,
Sahin Huseyin,
Erdem Ali Fuat
Publication year - 2014
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201301105
Subject(s) - naproxen , chromatography , derivatization , chemistry , calibration curve , extraction (chemistry) , ibuprofen , pharmacokinetics , gas chromatography–mass spectrometry , human plasma , gas chromatography , detection limit , high performance liquid chromatography , mass spectrometry , pharmacology , medicine , alternative medicine , pathology
This paper describes a GC–MS method for the determination of naproxen in human plasma. Naproxen and internal standard ibuprofen were extracted from plasma using a liquid–liquid extraction method. Derivatization was carried out using N ‐methyl‐ N ‐(trimethylsilyl)trifluoroacetamide. The calibration curve was linear between the concentration range of 0.10–5.0 μg/mL. Intra‐ and interday precision values for naproxen in plasma were <5.14, and accuracy (relative error) was better than 4.67%. The extraction recoveries of naproxen from human plasma were between 93.0 and 98.9%. The LOD and LOQ of naproxen were 0.03 and 0.10 μg/mL, respectively. Also, this assay was applied to determine the pharmacokinetic parameters of naproxen in six healthy Turkish volunteers who had been given 220 mg naproxen.