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Determination of fusaric acid in maize using molecularly imprinted SPE clean‐up
Author(s) -
Appell Michael,
Jackson Michael A.,
Wang Lijuan C.,
Ho CheHin,
Mueller Anja
Publication year - 2014
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201301065
Subject(s) - molecularly imprinted polymer , methacrylic acid , chemistry , molecular imprinting , picolinic acid , chromatography , methacrylate , fusaric acid , solid phase extraction , polymer , detection limit , monomer , organic chemistry , selectivity , fusarium , horticulture , biology , catalysis
A new LC method to detect fusaric acid ( FA ) in maize is reported based on a molecularly imprinted SPE clean‐up using mimic‐templated molecularly imprinted polymers. Picolinic acid was used as a toxin analog for imprinting polymers during a thermolytic synthesis. Both acidic and basic functional monomers were predicted to have favorable binding interactions by MP2 ab initio calculations. Imprinted polymers synthesized with methacrylic acid or 2‐dimethylaminoethyl methacrylate exhibited imprinting effects in SPE analysis. FA levels were determined using RP ion‐pairing chromatography with diode‐array UV detection and tetrabutylammonium hydrogen sulfate in the mobile phase. A method was developed to detect FA in maize using molecularly imprinted SPE analysis within the range of 1–100 μg/g with recoveries between 83.9 and 92.1%.