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Ultrasound/microwave‐assisted extraction and comparative analysis of bioactive/toxic indole alkaloids in different medicinal parts of G elsemium elegans Benth by ultra‐high performance liquid chromatography with MS / MS
Author(s) -
Li Yu,
Zeng RongJie,
Lu Qing,
Wu ShuiSheng,
Chen JianZhong
Publication year - 2014
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201300975
Subject(s) - chemistry , chromatography , formic acid , extraction (chemistry) , high performance liquid chromatography , indole test , electrospray , methanol , solid phase extraction , organic chemistry
Indole alkaloids are the main bioactive/toxic components in G elsemium elegans B enth. To determine the distribution and contents of indole alkaloids in its different medicinal parts, a novel and rapid method using ultra‐high performance LC ( UPLC ) with MS / MS has been established and validated with an optimized ultrasound/microwave‐assisted extraction method. Four constituents, namely, humantenidine, humantenmine, gelsemine, and koumine, were simultaneously determined in 6 min. Chromatographic separation was achieved on an ultra‐high performance LC BEH C 18 column with a gradient mobile phase consisting of methanol and water (containing 0.1% formic acid both in methanol and water) at a flow rate of 0.3 mL/min. The detection was performed on a triple quadrupole electrospray MS / MS by positive ion multiple‐reaction monitoring mode. All the analytes showed good linearity ( r ≥ 0.9934) within a concentration range from 0.1–25 μg/mL with a LOQ of 25–50 ng/mL. The overall intra‐ and intervariations of four components were <4.7% with an accuracy of 97.3–101.3%. The analysis results showed that there were remarkable differences in the distribution and contents of four chemical markers in the roots, stems, and leaves of G . elegans B enth. The findings can provide necessary and meaningful information for the rational utilization of its resources.