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Rapid analysis of aflatoxins B 1 , B 2 , and ochratoxin A in rice samples using dispersive liquid–liquid microextraction combined with HPLC
Author(s) -
Lai XianWen,
Sun DaiLi,
Ruan ChunQiang,
Zhang He,
Liu ChengLan
Publication year - 2014
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201300970
Subject(s) - aflatoxin , chromatography , ochratoxin a , mycotoxin , ochratoxin , extraction (chemistry) , chemistry , solvent , high performance liquid chromatography , detection limit , enrichment factor , derivatization , food science , organic chemistry
A novel, simple, and rapid method is presented for the analysis of aflatoxin B 1 , aflatoxin B 2 , and ochratoxin A in rice samples by dispersive liquid–liquid microextraction combined with LC and fluorescence detection. After extraction of the rice samples with a mixture of acetonitrile/water/acetic acid, mycotoxins were rapidly partitioned into a small volume of organic solvent (chloroform) by dispersive liquid–liquid microextraction. The three mycotoxins were simultaneously determined by LC with fluorescence detection after precolumn derivatization for aflatoxin B 1 and B 2 . Parameters affecting both extraction and dispersive liquid–liquid microextraction procedures, including the extraction solvent, the type and volume of extractant, the volume of dispersive solvent, the addition of salt, the pH and the extraction time, were optimized. The optimized protocol provided an enrichment factor of approximately 1.25 and with detection of limits (0.06–0.5 μg/kg) below the maximum levels imposed by current regulations for aflatoxins and ochratoxin A . The mean recovery of three mycotoxins ranged from 82.9–112%, with a RSD less than 7.9% in all cases. The method was successfully applied to measure mycotoxins in commercial rice samples collected from local supermarkets in C hina.