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Green chromatographic fingerprinting: A n environmentally friendly approach for the development of separation methods for fingerprinting complex matrices
Author(s) -
Funari Cristiano Soleo,
Carneiro Renato Lajarim,
Andrade André Marques,
Hilder Emily Frances,
Cavalheiro Alberto José
Publication year - 2014
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201300955
Subject(s) - fingerprint (computing) , chromatography , chemistry , chromatographic separation , metric (unit) , high performance liquid chromatography , computer science , artificial intelligence , engineering , operations management
A chromatographic fingerprint is a comprehensive method that reveals the distinctive pattern of peaks across the chromatogram for a given sample. It is considered an effective strategy to assess the identity and quality of herbal materials, as well as for the control of the quality of their derived products. HPLC is the most employed technique for these purposes and it is used routinely for quality control in industry. Hence, its impact on the environment should not be neglected. This work provides a rational and generic procedure to qualitatively fingerprint complex matrices. Resource‐ and time‐saving experimental designs were selected; an alternative safer organic solvent was tested and a time‐saving and innovative response entitled the green chromatographic fingerprinting response was developed and employed. This procedure was applied in the development of chromatographic fingerprints for extracts of B auhinia forficata and Casearia sylvestris . Moreover, the response proposed here can be combined with a complementary metric available in the literature to compare methods using different solvents. According to this, the chromatographic fingerprints developed here using ethanol as the organic solvent provided a performance better than that of reference methods in which more harmful acetonitrile or methanol were employed.