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Differential pharmacokinetics and the brain distribution of morphine and ephedrine constitutional isomers in rats after oral administration with Keke capsule using rapid‐resolution LC–MS/MS
Author(s) -
Song Yonggui,
Su Dan,
Lu Tulin,
Mao Chunqin,
Ji De,
Liu Yali,
Wei Binbin,
Fan Ronghua
Publication year - 2014
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201300886
Subject(s) - pharmacokinetics , pseudoephedrine , ephedrine , capsule , chemistry , morphine , pharmacology , oral administration , chromatography , distribution (mathematics) , theophylline , anesthesia , medicine , mathematical analysis , botany , mathematics , biology
Opioid and ephedra alkaloids known as the active ingredients for K eke capsule, which is used to treat coughs and bronchial asthma, could have potential adverse effects on the central nervous system. Therefore, an efficient, sensitive rapid‐resolution LC – MS / MS method for the simultaneous determination of morphine, ephedrine, and pseudoephedrine in rat plasma and brain tissue homogenate has been developed. The method was validated in the plasma and brain tissue samples, showed good linearity over a wide concentration range ( r 2 > 0.99). The intra‐ and interday assay variability was less than 15% for all analytes, and the accuracy was between −8.8 and 5.7%. The study provided the pharmacokinetics profiles and the brain regional distribution of the three active alkaloids after oral administration of K eke capsule. The results also indicated that significant difference in pharmacokinetics parameters of the epimers was observed between ephedrine and pseudoephedrine.