Premium
Quality control method for commercially available wild J ujube leaf tea based on HPLC characteristic fingerprint analysis of flavonoid compounds
Author(s) -
Zhang Rutan,
Chen Junhui,
Shi Qian,
Li Zhaoyong,
Peng Zhengyun,
Zheng Li,
Wang Xiaoru
Publication year - 2014
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201300841
Subject(s) - flavonoid , chromatography , fingerprint (computing) , high performance liquid chromatography , chemistry , quality assessment , evaluation methods , computer science , organic chemistry , artificial intelligence , engineering , reliability engineering , antioxidant
Flavonoids are the main active components of natural medicinal plants with many physiological functions. In this study, an HPLC fingerprinting method based on the distribution and relative amount of 11 bioactive flavonoids was established for the quality evaluation of commercially available wild Jujube leaf tea ( JLT ) from China. Separation of the crude flavonoid extract was achieved on a column filled with C 18 material with a high carbon content. The flavonoids in wild JLT were identified based on UV spectroscopy and accurate mass measurements by TOF ‐ MS . Twenty‐one batches of practical samples collected from different habitats were analyzed by using the developed HPLC method to construct the HPLC characteristic fingerprint of wild JLT . Then, combined with clustering and similarity analyses, the HPLC characteristic fingerprint was used for the authentication and quality evaluation of commercial wild JLT . Results indicated that the proposed HPLC characteristic fingerprint reflected the inherent characteristics of wild JLT collected from different regions. Authenticity identification and quality control of commercially available wild Jujube tea were achieved based on the HPLC characteristic fingerprint analysis. This new approach to bioactive component profiling provided a promising reference method for the quality evaluation of commercial wild flower and plant tea.