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Combined use of hydroxypropyl‐β‐cyclodextrin and ionic liquids for the simultaneous enantioseparation of four azole antifungals by CE and a study of the synergistic effect
Author(s) -
Zhao Min,
Cui Yan,
Yu Jia,
Xu Shuying,
Guo Xingjie
Publication year - 2014
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201300831
Subject(s) - chemistry , azole , ionic liquid , cyclodextrin , enantiomer , chromatography , ammonium chloride , capillary electrophoresis , nuclear chemistry , organic chemistry , antifungal , medicine , dermatology , catalysis
A CE method employing a dual system of hydroxypropyl‐β‐cyclodextrin ( HP ‐β‐ CD ) and ionic liquids ( IL s) has been developed for the simultaneous enantioseparation of four azole antifungals for the first time. In this study, three different types of IL s were employed as modifiers and among them dodecyl trimethyl ammonium chloride was found to be the most effective. The effects of the concentration, cations, and anions of IL s on the enantioseparation were investigated. With the developed dual system, all the enantiomers were well separated in resolutions of 3.8, 3.5, 2.8, and 2.5 for miconazole, econazole, ketoconazole, and itraconazole, respectively. The interactions between dodecyl trimethyl ammonium chloride and HP ‐β‐ CD were also studied using a neutral polyacrylamide coated capillary and 1 H NMR spectroscopy to further explore the synergistic effect involved. It was found that IL s improved the enantioseparation not only by changing the EOF , but also by interactions with HP ‐β‐ CD that could change its ability of forming inclusion complex with the enantiomers.