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Ultra‐fast LC – ESI ‐ MS / MS method for the simultaneous determination of six highly toxic A conitum alkaloids from Aconiti kusnezoffii radix in rat plasma and its application to a pharmacokinetic study
Author(s) -
Liu Jingjing,
Li Qing,
Yin Yidi,
Liu Ran,
Xu Huarong,
Bi Kaishun
Publication year - 2014
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201300775
Subject(s) - aconitine , aconitum , chemistry , chromatography , extraction (chemistry) , analyte , radix (gastropod) , pharmacokinetics , alkaloid , pharmacology , organic chemistry , medicine , botany , biology
A fast, sensitive, and efficient ultra‐fast LC – ESI ‐ MS / MS method was developed for the simultaneous quantitation of six highly toxic A conitum alkaloids, that is, aconitine, mesaconitine, hypaconitine, benzoylaconine, benzoylmesaconine, and benzoylhypaconine, in rat plasma after oral administration of crude ethanol extracts from A coniti kusnezoffii radix by ultrasonic extraction, reflux extraction for 1 h, and reflux extraction for 3 h, respectively. The separation of six A conitum alkaloids and aminopyrine (internal standard) was performed on an InertSustain® C 18 column, and the quantification of the analytes was performed on a 4000Q ultra‐fast LC – MS / MS system with turbo ion spray source in the positive ion and multiple‐reaction monitoring mode. Absolute recoveries ranged within 65.06–85.1% for plasma samples. The intra‐ and interday precision and accuracy of analytes were satisfactory. The methods were validated with sensitivity reaching the lower LOQ for aconitine, mesaconitine, hypaconitine, benzoylaconine, benzoylmesaconine, and benzoylhypaconine, which were 0.025, 0.025, 0.050, 0.025, 0.025, and 0.100 ng/mL, respectively. The method was successfully applied to a pharmacokinetic study of six A conitum alkaloids in rat plasma after oral administration of crude ethanol extracts from the raw root of A conitum kusnezoffii R eichb. by three different extraction processes.