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Determination of free formaldehyde in vaccines and biological samples using solid‐phase microextraction coupled to GC – MS
Author(s) -
Zali Sara,
Jalali Fahimeh,
Eshaghi Ali,
Shamsipur Mojtaba
Publication year - 2013
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201300771
Subject(s) - solid phase microextraction , chromatography , detection limit , formaldehyde , chemistry , extraction (chemistry) , solid phase extraction , gas chromatography–mass spectrometry , analytical chemistry (journal) , mass spectrometry , organic chemistry
A headspace solid‐phase microextraction method coupled to GC – MS was successfully developed for the trace determination of formaldehyde in veterinary bacterial and human vaccines, and diphtheria–tetanus antigen. The formaldehyde was derivatized by means of the H antzsch reaction prior to extraction and subsequent determination. Three different types of solid‐phase microextraction fibers, polar, and nonpolar poly(dimethylsiloxane) and polyethylene glycol were prepared by using a sol–gel technique. The effects of different parameters such as type of fiber coating, extraction time and temperature, desorption conditions, agitation rate, and salt effect were investigated. Under the optimized conditions, the detection limit of the method was 979 ng/L using the selected ion‐monitoring mode. The interday and intraday precisions of the developed method under the optimized conditions were below 13%, and the method shows linearity in the range of 1.75–800 μg/L with a correlation coefficient of 0.9963. The optimized method was applied to the determination of formaldehyde from some biological products. The results were satisfactory compared to the standard method.