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Preparation and application of hollow molecularly imprinted polymers with a super‐high selectivity to the template protein
Author(s) -
Chen Yang,
He XiWen,
Mao Jie,
Li WenYou,
Zhang YuKui
Publication year - 2013
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201300709
Subject(s) - molecularly imprinted polymer , polymer , molecular imprinting , selectivity , polymerization , lysozyme , adsorption , materials science , covalent bond , chemical engineering , molecule , nanotechnology , chemistry , polymer chemistry , organic chemistry , catalysis , biochemistry , engineering
Protein‐imprinted polymers with hollow cores that have a super‐high imprinting factor were prepared by etching the core of the surface‐imprinted polymers that used silica particles as the support. Lysozyme as template was modified onto the surface of silica particles by a covalent method, and after polymerization and the removal of template molecules, channels through the polymer layer were formed, which allowed a single‐protein molecule to come into the hollow core and attach to the binding sites inside the polymer layer. The adsorption experiments demonstrated that the hollow imprinted polymers had an extremely high binding capacity and selectivity, and thus a super‐high imprinting factor was obtained. The as‐prepared imprinted polymers were used to separate the template lysozyme from egg white successfully, indicating its high selectivity and potential application in the field of separation of protein from real samples.