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Regeneration of a silica monolithic rod column using harsh methods followed by firm thermodynamic and kinetic validation
Author(s) -
Samuelsson Jörgen,
Cavazzini Alberto,
Shalliker Ross Andrew,
Fornstedt Torgny
Publication year - 2014
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201300683
Subject(s) - adsorption , inlet , monolithic hplc column , monolith , column (typography) , chemistry , chromatography , kinetic energy , materials science , analytical chemistry (journal) , structural engineering , high performance liquid chromatography , catalysis , geology , organic chemistry , physics , connection (principal bundle) , quantum mechanics , engineering , geomorphology
In this study, a numerical tool is introduced—based on thermodynamic and kinetic separation theory—for validating the regeneration of monolithic rod columns after cutting their inlet sections. A long‐used RP ‐18e monolithic column was deemed to be unfit for further coffee analysis because of poor separation performance. The columns brownish inlet section was physically removed with a lathe, leaving a clean white inlet section. The original and regenerated columns were extensively analyzed and compared using numerical tools for processing adsorption data. The perturbation peak method was used to measure the adsorption isotherm of phenol on the original and regenerated monolith and the adsorption energy distributions were calculated for identifying any change in the degree of heterogeneity. Although peak shapes improved considerably after regeneration, no significant differences were found in the detailed characterization of the processed adsorption data between the original column and the regenerated one. This indicates that the removal of a section of the monolithic bed can be undertaken without damaging the column and columns in which their inlet head sections are contaminated may still function with normal adsorption behavior. In addition, the combined thermodynamic and kinetic methodology could accurately be used to evaluate any regeneration method of columns.