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Identification of regioisomers and enantiomers of triacylglycerols in different yeasts using reversed‐ and chiral‐phase LC – MS
Author(s) -
Řezanka Tomáš,
Kolouchová Irena,
Čejková Alena,
Cajthaml Tomáš,
Sigler Karel
Publication year - 2013
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201300657
Subject(s) - structural isomer , palmitoleic acid , chemistry , enantiomer , yarrowia , chromatography , propanoic acid , atmospheric pressure chemical ionization , stereochemistry , fatty acid , yeast , organic chemistry , chemical ionization , linoleic acid , biochemistry , ionization , ion
LC with atmospheric pressure chemical ionization (ACPI) MS with RP and chiral phase was used for separation of triacylglycerols ( TAG s) from yeasts of the genera C andida , K luyveromyces , R hodotorula , S accharomyces , T orulospora , T richosporon , and Y arrowia . Chiral LC – APCI ‐ MS is based on using two columns in series packed with a 3,5‐dimethylphenyl carbamate modified β‐cyclodextrin chiral phase. All regioisomers and enantiomers of TAG s containing one to five double bonds were separated. Molecular species of TAG s, i.e. regioisomers and enantiomers, were identified and quantified by MS / MS . Among the 94 identified TAG s, the most abundant were triolein, oleopalmitoleoolein, and dipalmitoleoolein. In strains producing palmitoleic acid in amounts >25% of total fatty acids ( FA s), this acid, or unsaturated FA is bound in sn ‐1. In strains containing palmitoleic acid at 10–25% total FA s this acid is mainly bound in sn ‐3, saturated FA being bound in sn ‐1. Strains containing <10% palmitoleic acid form preferentially symmetrical TAG s.