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Normal‐phase high‐performance counter‐current chromatography for the fractionation of dissolved organic matter from a freshwater source
Author(s) -
Sandron Sara,
Nesterenko Pavel N.,
McCaul Margaret V.,
Kelleher Brian,
Paull Brett
Publication year - 2014
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201300634
Subject(s) - fractionation , chemistry , chromatography , countercurrent chromatography , absorbance , phase (matter) , dissolved organic carbon , ethyl acetate , fraction (chemistry) , high performance liquid chromatography , alicyclic compound , analytical chemistry (journal) , solvent , environmental chemistry , organic chemistry
Normal‐phase high‐performance counter‐current chromatography ( HPCCC ) is used to obtain a preliminary fractionation of components in dissolved organic matter ( DOM ) from a freshwater source. The HPCCC solvent system involved a normal‐phase approach with water/methanol (1:1) as the lower stationary phase and hexane/ethyl acetate (1:1) as the upper mobile phase. The critical experiment parameters were optimised: revolution speed 1800 rpm and flow rate 0.15 mL/min. Under these conditions 50 μL of a 0.50 mg/mL DOM solution was loaded. The detection wavelength was monitored at 330 nm in order to isolate the main portion of DOM , which includes substances such as carboxyl‐rich alicyclic molecules. By optimising this system it was possible to isolate materials that, according to GC – MS , can be related to molecules with an analogous structural background. Where fraction analysis was not suitable for GC – MS , RP ‐ HPLC with UV absorbance detection was used, showing unique chromatograms for each fraction at both 210 and 330 nm.