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Ultra high performance liquid chromatography with ion‐trap TOF ‐ MS for the fast characterization of flavonoids in C itrus bergamia juice
Author(s) -
Sommella Eduardo,
Pepe Giacomo,
Pagano Francesco,
Tenore Gian Carlo,
Dugo Paola,
Manfra Michele,
Campiglia Pietro
Publication year - 2013
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201300591
Subject(s) - chromatography , chemistry , ion trap , mass spectrometry , repeatability , high performance liquid chromatography , resolution (logic) , flavonoid , analytical chemistry (journal) , quadrupole ion trap , particle size , time of flight mass spectrometry , ion , biochemistry , organic chemistry , artificial intelligence , computer science , ionization , antioxidant
We have developed a fast ultra HPLC with ion‐trap TOF ‐ MS method for the analysis of flavonoids in C itrus bergamia juice. With respect to the typical methods for the analysis of these matrices based on conventional HPLC techniques, a tenfold faster separation was attained. The use of a core–shell particle column ensured high resolution within the fast analysis time of only 5 min. Unambiguous determination of flavonoid identity was obtained by the employment of a hybrid ion‐trap TOF mass spectrometer with high mass accuracy (average error 1.69 ppm). The system showed good retention time and peak area repeatability, with maximum RSD % values of 0.36 and 3.86, respectively, as well as good linearity ( R 2 ≥ 0.99). Our results show that ultra HPLC can be a useful tool for ultra fast qualitative/quantitative analysis of flavonoid compounds in citrus fruit juices.