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Liquid chromatography of synthetic polymers under critical conditions of enthalpic interactions 4: Sample recovery
Author(s) -
Šišková Alena,
Macová Eva,
Corradini Danilo,
Berek Dušan
Publication year - 2013
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201300545
Subject(s) - elution , molar mass , chromatography , polymer , chemistry , column chromatography , tetrahydrofuran , methacrylate , methyl methacrylate , gel permeation chromatography , toluene , sample preparation , organic chemistry , monomer , solvent
Reduced sample recovery is a frequent feature of LC of macromolecules under critical conditions of enthalpic interactions ( LC CC ). Several methods of assessment of LC CC sample recovery are compared. A novel approach is based on an online combination of the. The LC CC column with a noninteractive SEC column. It provides not only the amount but also the molar mass of the eluted/withheld polymer. The procedure was tested with poly(methyl methacrylate), bare silica gel column packings, and “critical eluent” tetrahydrofuran/toluene. It was shown that macromolecules with higher molar masses were preferentially trapped within the LC CC column packing so that the eluted part of the sample was no longer representative. The incomplete polymer elution can make the LC CC polymer analyses susceptible to significant experimental errors.