Enantioseparation of tartaric acid by ligand‐exchange capillary electrophoresis using contactless conductivity detection | Zendy

Knob Radim | Zendy; Petr Jan | Zendy; Ševčík Juraj | Zendy; Maier Vítězslav | Zendy

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Enantioseparation of tartaric acid by ligand‐exchange capillary electrophoresis using contactless conductivity detection

Author(s) -

Knob Radim,

Petr Jan,

Ševčík Juraj,

Maier Vítězslav

Publication year - 2013

Publication title -

journal of separation science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.72

H-Index - 102

eISSN - 1615-9314

pISSN - 1615-9306

DOI - 10.1002/jssc.201300507

Subject(s) - tartaric acid , chemistry , capillary electrophoresis , chromatography , enantiomer , detection limit , counterion , conductivity , resolution (logic) , conductometry , hydrochloric acid , analytical chemistry (journal) , citric acid , inorganic chemistry , ion , organic chemistry , artificial intelligence , computer science

A method for the determination of tartaric acid enantiomers using CE with contactless conductivity detection has been developed. Cu( II ) as a central metal ion together with l ‐hydroxyproline were used as a chiral selector, the BGE was composed of 7 mM CuCl 2, 14 mM trans ‐4‐hydroxy‐ l ‐proline, and 100 mM ε‐aminocaproic acid; the pH was adjusted to 5 by hydrochloric acid. Separation with a resolution of 1.9 was achieved in 9 min in a polyacrylamide‐coated capillary to suppress the EOF. Various counterions of the BGE were studied, and migration order reversal was achieved when switching from ε‐aminocaproic acid to l ‐histidine. With detection limits of about 20 μM, the method was applied to the analysis of wine and grape samples; only l ‐tartaric acid was found.

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