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A direct sensitivity comparison between flow‐modulated comprehensive 2D and 1D GC in untargeted and targeted MS‐based experiments
Author(s) -
Tranchida Peter Q.,
Franchina Flavio A.,
Zoccali Mariosimone,
Bonaccorsi Ivana,
Cacciola Francesco,
Mondello Luigi
Publication year - 2013
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201300423
Subject(s) - two dimensional gas , chromatography , chemistry , sensitivity (control systems) , mass spectrometry , analytical chemistry (journal) , triple quadrupole mass spectrometer , flow (mathematics) , volumetric flow rate , column (typography) , process engineering , selected reaction monitoring , computer science , tandem mass spectrometry , electronic engineering , physics , engineering , mechanics , telecommunications , frame (networking)
The present contribution is focused on the measurement of the analytical sensitivity attained in untargeted/targeted MS/MS experiments, performed using flow‐modulator comprehensive 2D and 1D GC. The comprehensive 2D experiment was performed by diverting part of the high flow (circa 80%) to flush the accumulation loop (about 28 mL/min) to waste, to reduce the gas flow entering the ion source. 1D analyses were performed through: (i) unmodulated and (ii) single column applications. An equivalent temperature program was applied in the modulated and unmodulated analyses, while a faster one was employed in the single column one. In all application types, the (same) triple quadrupole instrument was operated in the full‐scan and multiple reaction monitoring modes. A genuine sweet orange oil and the same sample spiked with 20 phytosanitary compounds were employed to reach the research objective. The results highlight the problems related to the flow modulation–MS combination. Specifically, it was found that sensitivity was on average three to four times higher in unmodulated and optimized single‐column applications.