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Evaluation of stationary phases based on silica hydride for the analysis of drugs of abuse
Author(s) -
Pesek Joseph J.,
Matyska Maria T.,
Kim Amy M.
Publication year - 2013
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201300377
Subject(s) - formic acid , ammonium formate , chromatography , chemistry , hydride , methanol , isobaric process , organic chemistry , hydrogen , physics , thermodynamics
Several stationary phases based on silica hydride were evaluated as possible separation media for drugs of abuse. In the RP mode both a C 18 and a phenyl column were tested using ACN /water and methanol/water mobile phases with formic acid or ammonium formate as modifiers. Detection was performed using MS , so separation of isobaric species was a factor in selecting the column, mobile phase composition, and gradient. A number of sample preparation procedures were also included as part of determining the most appropriate experimental protocol on these types of stationary phases. Morphine was used as a model for developing a protocol that would be suitable for the analysis of hydrophilic drugs.