Premium
Usefulness of the chaotropic effect in sample preparation for chromatographic analysis of acidic xenobiotics in human plasma
Author(s) -
Flieger Jolanta,
CzajkowskaŻelazko Anna
Publication year - 2013
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201300351
Subject(s) - chromatography , chemistry , chaotropic agent , detection limit , extraction (chemistry) , calibration curve , sample preparation , elution , ionic liquid , methanol , chloride , solid phase extraction , organic chemistry , catalysis
In this study a new RP‐HPLC with photo‐diode array detection method for the determination of ibuprofen (( RS )‐2‐(4‐isobutylphenyl)propionic acid) in human plasma samples was developed. Samples were prepared by SPE and analyzed by an isocratic elution mode over a C 18 column using 80% methanol. A novel sample pretreatment method, based on the addition of ionic liquids possessing chaotropic ions to small human plasma sample (100 μL), was elaborated. 1‐Butyl‐3‐methylimidazolium chloride and 1‐butyl‐3‐methylimidazolium tetrafluoroborate (BMIM BF 4 ) were tested from the point of view of extraction yield. Quantification was based on calibration curve applying diclofenac as the internal standard. Owing to dilution of plasma sample by 2 mM aqueous solution of BMIM BF 4 before SPE, appropriate sample purification and extraction yields higher than 95% with precision lower than 2% can be achieved. Linear coefficients of correlation ( r 2 ) were >0.99 in the range of 0.3–5 μg/mL ibuprofen concentration in plasma. The limit of quantification was 65 ng/mL and the detection limit for ibuprofen was 19.5 ng/mL.