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High‐performance anion‐exchange chromatography coupled with diode array detection for the determination of dencichine in P anax notoginseng and related species
Author(s) -
Qiao ChunFeng,
Liu XiaoMei,
Cui XiuMing,
Hu DeJun,
Chen YiWen,
Zhao Jing,
Li ShaoPing
Publication year - 2013
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201300334
Subject(s) - panax notoginseng , chromatography , repeatability , calibration curve , chemistry , detection limit , high performance liquid chromatography , chromatography detector , elution , linearity , analytical chemistry (journal) , medicine , alternative medicine , pathology , physics , quantum mechanics
A high‐performance anion‐exchange chromatography coupled with diode array detection method was developed for the determination of dencichine in Panax notoginseng and related species. The analysis was performed on an Eprogen Synchropak WAX column (4.6 × 250 mm, 6 μm) with 50 mM NaH 2 PO 4 aqueous solution isocratic elution. The method was validated in terms of linearity, sensitivity, precision, stability, and accuracy. It was found that the calibration curve for dencichine showed good linearity ( R 2 = 0.9999) within the test range. The LOD and LOQ were 0.77 and 3.06 ng, respectively. The RSD for intra‐ and interday repeatability was 0.2 and 0.5%, respectively. The test solution of dencichine is stable at least for three days at room temperature and for seven days at 4°C. The mean recovery of dencichine was 102.0%. The established method was successfully applied to determine dencichine in the raw root of P. nogoginseng , P. ginseng , and P. quinquefolium as well as the steamed root of P. notoginseng . Compared with previous reports, this method is sensitive, selective, and accurate, which is helpful to evaluate the quality of P. notoginseng and related species.