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SPE and purification of DNA using magnetic particles
Author(s) -
Rittich Bohuslav,
Španová Alena
Publication year - 2013
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201300331
Subject(s) - biomolecule , magnetic nanoparticles , magnetite , superparamagnetism , paramagnetism , polymer , magnetite nanoparticles , magnetic separation , nanotechnology , materials science , chemistry , solid phase extraction , nanoparticle , extraction (chemistry) , chromatography , magnetization , magnetic field , organic chemistry , physics , quantum mechanics , metallurgy
Superparamagnetic particles have been attractive for molecular diagnostics and analytical chemistry applications due to their unique magnetic properties and their ability to interact with various biomolecules of interest. This paper presents a critical overview of magnetic nano ‐ and microparticles used as a solid phase for extraction and purification of DNAs. The mechanisms of DNA binding to the surface of functionalised magnetic particles are described. The most widely used materials including silica supports, organic polymers and other materials, mostly containing magnetite or paramagnetic metallic elements are reviewed. The main application areas of magnetic particles for DNA separation are briefly described.