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Separation and characterization of sucrose esters from O riental tobacco leaves using accelerated solvent extraction followed by SPE coupled to HPLC with ion‐trap MS detection
Author(s) -
Zhu Hongying,
Feng Yonggang,
Yang Jun,
Pan Wenjie,
Li Zhanghai,
Tu Yonggao,
Zhu Xiaolan,
Huang Guangming
Publication year - 2013
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201300294
Subject(s) - chromatography , extraction (chemistry) , chemistry , ion trap , high performance liquid chromatography , solid phase extraction , solvent , electrospray , mass spectrometry , electrospray ionization , biochemistry
Sucrose esters (SEs) were successfully extracted from O riental tobacco leaves using a new methodology based on accelerated solvent extraction followed by hydrophilic–lipophilic balanced cartridge cleanup step. The SEs were detected by HPLC with ion‐trap MS detection using an electrospray interface operated in the positive ion mode. This methodology combines the high efficiency of extraction provided by a pressurized fluid and the highly sensitive characterization offered by ion‐trap MS . Under the optimized conditions, 14 SEs were first identified among a total of 23 SEs found in O riental tobacco leaves. Under the same conditions, only four new SEs were extracted by using traditional ultrasound‐assisted extraction and liquid–solid extraction methods. The present method might be potentially useful in high‐efficiency extraction and sensitive characterization of SEs from complex matrices such as tobacco leaves.