Premium
Preparation and characterization of octadecyl acrylate monoliths for capillary electrochromatography by photochemical, thermal, and chemical initiation †
Author(s) -
EscrigDoménech Aarón,
TenDoménech Isabel,
SimóAlfonso Ernesto F.,
HerreroMartínez José M.
Publication year - 2013
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201300288
Subject(s) - capillary electrochromatography , chemistry , acrylate , solvent , polymerization , electrochromatography , irradiation , peroxide , capillary action , monolithic hplc column , chemical engineering , chromatography , high performance liquid chromatography , materials science , monomer , polymer , organic chemistry , stationary phase , physics , nuclear physics , engineering , composite material
Monolithic stationary phases based on octadecyl acrylate for CEC using different initiating systems (UV irradiation, thermal, and chemical initiation) in the presence of lauroyl peroxide as initiator were synthesized. For each initiation mode, the influence of the porogenic solvent composition on both the morphological and electrochromatographic properties of the resulting monoliths was investigated. Under optimal conditions, excellent efficiencies for the photochemically and chemically polymerized monoliths (minimum plate heights of 6.9–10.7 and 6.5–12.6 μm, respectively) were achieved. Thermally initiated columns gave lower efficiency values, permeabilities, and longer analysis times compared to these initiating systems. The produced monolithic stationary phases were evaluated in terms of reproducibility and gave RSD values below 9.2, 10.6, and 9.8% for UV, thermally, and chemically initiated columns, respectively.