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Properties of two amide‐based hydrophilic interaction liquid chromatography columns
Author(s) -
Kalíková Květa,
Kozlík Petr,
Gilar Martin,
Tesařová Eva
Publication year - 2013
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201300232
Subject(s) - amide , selectivity , chromatography , chemistry , hydrophilic interaction chromatography , analyte , high performance liquid chromatography , characterization (materials science) , materials science , organic chemistry , nanotechnology , catalysis
Hydrophilic interaction liquid chromatography is a separation technique suitable for the separation of moderately and highly polar compounds. Various stationary phases ( SP s) for hydrophilic interaction liquid chromatography are commercially available. While the SPs based on the same type of ligand are available from different providers, they can display a distinct retention characteristics and separation selectivity. The current work is focused on characterization and comparison of the separation systems of two amide‐based HPLC columns from two producers, i.e. XB ridge Amide column and TSK gel A mide‐80 column. Several characterization procedures (tests) were used to investigate the differences between these columns. The chromatographic behavior of selected analytes indicates that multimodal interactions are responsible for retention and separation on these columns. Multiple testing approaches were used in order to reveal subtle differences between the SP s. Both amide‐based columns showed certain differences in retention, selectivity, and plate counts. Based on the tests used in this study, we conclude that the investigated columns provide a different degree of H ‐bonding interactions.