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Enantioseparation of chiral aromatic acids by multiple dual mode counter‐current chromatography using hydroxypropyl‐β‐cyclodextrin as chiral selector
Author(s) -
Tong Shengqiang,
Zheng Ye,
Yan Jizhong
Publication year - 2013
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201300193
Subject(s) - chemistry , chromatography , countercurrent chromatography , elution , resolution (logic) , chiral resolution , cyclodextrin , phosphate buffered saline , enantiomer , high performance liquid chromatography , stereochemistry , artificial intelligence , computer science
This work concentrates on extending the utilization of multiple dual mode ( MDM ) counter‐current chromatography in chiral separations. Two aromatic acids, 2‐(6‐methoxy‐2‐naphthyl)propionic acid ( NAP ) and 2‐phenylpropionic acid (2‐ PPA ), were enantioseparated by MDM counter‐current chromatography using hydroxypropyl‐β‐cyclodextrin (HP‐β‐CD) as chiral selector. The two‐phase solvent systems consisting of n ‐hexane/ethyl acetate 0.1 mol/L phosphate buffer pH 2.67 containing 0.1 mol/L HP‐β‐ CD (7.5:2.5:10 for NAP and 7:3:10 for 2‐ PPA , v/v/v) were used. Conventional MDM and modified MDM were compared according to peak resolution under current separation mechanism. The influence of elution time after the first‐phase inversion and number of cycles for MDM were investigated. Peak resolution of NAP and 2‐ PPA increased from 0.62 to 1.05 and 0.72 to 0.84, respectively, using optimized MDM conditions. Being an alternative elution method for counter‐current chromatography, MDM elution greatly improved peak resolution in chiral separations.