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Solanidine isolation from Solanum tuberosum by centrifugal partition chromatography
Author(s) -
Attoumbré Jacques,
Giordanengo Philippe,
BaltoraRosset Sylvie
Publication year - 2013
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201300188
Subject(s) - chromatography , solanum tuberosum , chemistry , partition (number theory) , isolation (microbiology) , mathematics , biology , botany , combinatorics , bioinformatics
The aim of this investigation was the preparative isolation of solanidine (aglycone of the two main potato glycoalkaloids: α‐chaconine and α‐solanine) from fresh Solanum tuberosum (cv. P ompadour) material by implementing a new preparation scheme using centrifugal partition chromatography ( CPC ). A setup for obtaining solanidine by hydrolysis of the glycoalkaloids found in the skin and sprouts of S . tuberosum was first developed. Then its isolation was carried out by the development of CPC conditions: the solvent system used for separation was ethyl acetate/butanol/water in the ratio 42.5:7.5:50 v/v/v, 0.6 g of crude extract were separated with a 8 mL/min flow rate of mobile phase while rotating at 2500 rpm. A run yielded 98 mg of solanidine (86.7 % recovery from the crude extract) in a one‐step separation. The purity of the isolated solanidine was over 98%. Thus, CPC has proven to be the method of choice to get solanidine of very high purity from S . tuberosum biomass in large quantities.