Premium
Optimization of programmed‐temperature vaporization injection preparative capillary GC for compound specific radiocarbon analysis
Author(s) -
Zhang Xinyu,
Zhao Liang,
Wang Yexin,
Xu Yunping,
Zhou Liping
Publication year - 2013
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201300088
Subject(s) - chemistry , fatty alcohol , chromatography , boiling point , vaporization , volume (thermodynamics) , gas chromatography , complex matrix , capillary action , organic chemistry , materials science , physics , quantum mechanics , composite material
Preparative capillary GC ( PCGC ) is a powerful tool for the separation and purification of compounds from any complex matrix, which can be used for compound‐specific radiocarbon analysis. However, the effect of PCGC parameters on the trapping efficiency is not well understood. Here, we present a comprehensive study on the optimization of parameters based on 11 reference compounds with different physicochemical properties. Under the optimum conditions, the trapping efficiencies of these 11 compounds (including high‐boiling‐point n ‐hentriacontane and methyl lignocerate) are about 80% (60–89%). The isolation of target compounds from standard solutions, plant and soil samples demonstrates that our optimized method is applicable for different classes of compounds including n ‐alkanes, fatty acid esters, long‐chain fatty alcohol esters, polycyclic aromatic hydrocarbons (PAHs) and steranes. By injecting 25 μL in large volume injection mode, over 100 μg, high purity (>90%) target compounds are harvested within 24 h. The recovery ranges of two real samples are about 70% (59.9–83.8%) and about 83% (77.2–88.5%), respectively. Compared to previous studies, our study makes significant improvement in the recovery of PCGC , which is important for its wide application in biogeochemistry, environmental sciences, and archaeology.