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Simultaneous determination of 11 related impurities in propofol by gas chromatography/tandem mass spectrometry coupled with pulsed splitless injection technique
Author(s) -
Peng Ming,
Le Jian,
Yang Yongjian
Publication year - 2013
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201300075
Subject(s) - impurity , chemistry , chromatography , mass spectrometry , degradation (telecommunications) , propofol , gas chromatography–mass spectrometry , gas chromatography , detection limit , analytical chemistry (journal) , organic chemistry , medicine , telecommunications , computer science , pharmacology
A variety of related impurities, including starting materials, process impurities, and degradation products, can be detected in propofol. In this article, a sensitive and selective GC ‐ MS / MS method using pulsed splitless injection technique for the determination of 11 main related impurities in propofol in one chromatogram is investigated. This method is extensively validated for its linearity, recovery, precision, LOD , and LOQ , and is able to detect trace‐level related impurities ( LOD = 0.2–5.6 μg/g) in propofol bulk drug. Stressed tests proposed that oxidative degradation, photolytic degradation, and heat are the main causes for the formation of degradation products in propofol.