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Separation of peptides with an aqueous mobile phase by temperature‐responsive chromatographic column (J. Sep. Science 35'16)
Author(s) -
Dai Rongji,
Liu Zongjian,
Liang Yanli,
Geng Fangfang,
Ge Chen,
Ullah Kaleem,
Lv Fang,
Zhang Yukui,
Deng Yulin
Publication year - 2012
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201270122
Subject(s) - chemistry , chromatography , aqueous solution , phase (matter) , polymer , chromatographic separation , peptide , stationary phase , high performance liquid chromatography , organic chemistry , biochemistry
DOI: 10.1002/jssc.201200247 A thermoresponsive chromatographic material was prepared through a simple method and employed for the temperature‐modulated separation of peptides in an aqueous mobile phase. At 10°C, the grafted polymer chains are hydrophilic and in the form of extended coil. In this case, the peptides were unable to be separated and showed a single peak in chromatograms. In contrast, the polymer is hydrophobic and in the form of collapsed globule at 50°C. In this case, the peptides were completely separated because of the enhanced hydrophobic interaction of peptides and stationary phase.