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Simultaneous separation of water‐ and fat‐soluble vitamins in isocratic pressure‐assisted capillary electrochromatography using a methacrylate‐based monolithic column
Author(s) -
Yamada Hiroki,
Kitagawa Shinya,
Ohtani Hajime
Publication year - 2013
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201201191
Subject(s) - chromatography , ammonium formate , chemistry , capillary electrochromatography , elution , methacrylate , formic acid , fat soluble vitamin , monolithic hplc column , monolith , alkylbenzenes , acetonitrile , formate , methacrylic acid , high performance liquid chromatography , capillary electrophoresis , polymer , vitamin , copolymer , organic chemistry , biochemistry , benzene , catalysis
A method of simultaneous separation of water‐ and fat‐soluble vitamins using pressure‐assisted CEC with a methacrylate‐based capillary monolithic column was developed. In the proposed method, water‐soluble vitamins were mainly separated electrophoretically, while fat soluble‐ones were separated chromatographically by the interaction with a methacrylate‐based monolith. A mixture of six water‐soluble and four fat‐soluble vitamins was separated simultaneously within 20 min with an isocratic elution using 1 M formic acid (pH 1.9)/acetonitrile (30:70, v/v) containing 10 mM ammonium formate as a mobile phase. When the method was applied to a commercial multivitamin tablet and a spiked one, the vitamins were successfully analyzed, and no influence of the matrix contained in the tablet was observed.