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Reversal of elution order of N ‐(2,4‐dinitrophenyl)‐proline and N ‐(2,4‐dinitrophenyl)‐serine in HPLC by BSA chiral stationary phase
Author(s) -
Wang Qiuyun,
Xiong Yajin,
Lu Baozhu,
Fan Jun,
Zhang Sheng,
Zheng Shengrun,
Zhang Weiguang
Publication year - 2013
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201201165
Subject(s) - chemistry , chromatography , acetonitrile , dinitrophenyl , elution , enantiomer , high performance liquid chromatography , proline , amino acid , organic chemistry , biochemistry , antibody , immunology , biology
N ‐(2,4‐dinitrophenyl)‐proline and N ‐(2,4‐dinitrophenyl)‐serine were enantiomerically resolved on the BSA chiral stationary phase by HPLC in reversed‐phase mode. Effects of chromatographic conditions on enantioseparation and elution order have been investigated in detail. For these two samples, reversal of enantiomer elution order was observed by changing buffer pH, the content of acetonitrile, or alcohol modifiers in mobile phase, which is firstly reported in the BSA chiral stationary phase studies. More interestingly, combined effect between buffer pH and the content of acetonitrile was also observed. In addition, coelution range of enantiomers varied along with the content of acetonitrile in mobile phase.