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An interesting two‐phase solvent system and its use in preparative isolation of aconitines from aconite roots by counter‐current chromatography
Author(s) -
Han QuanBin,
Tang WaiLun,
Dong CaiXia,
Xu HongXi,
Jiang ZhiHong
Publication year - 2013
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201201079
Subject(s) - chemistry , aconitine , countercurrent chromatography , chromatography , chloroform , solvent , aconitum , ethyl acetate , dissolution , methanol , organic solvent , partition coefficient , column chromatography , organic chemistry , alkaloid , chemical engineering , engineering
Two‐phase solvent system plays crucial role in successful separation of organic compounds using counter‐current chromatography ( CCC ). An interesting two‐phase solvent system, composed of chloroform/ethyl acetate/methanol/water, is reported here, in which both phases contain sufficient organic solvents to balance their dissolving capacities. Adjusting the solvent system to get satisfactory partition coefficients ( K values) for target compounds becomes relatively simple. This solvent system succeeded in sample preparation of aconitine (8.07 mg, 93.69%), hypaconitine (7.74 mg, 93.17%), mesaconitine (1.95 mg, 94.52%) from raw aconite roots (102.24 mg, crude extract), benzoylmesaconine (34.79 mg, 98.67%) from processed aconite roots (400.01 mg, crude extract), and yunaconitine (253.59 mg, 98.65%) from a crude extract of Aconitum forrestii (326.69 mg, crude extract).