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Acid‐induced homogenous liquid‐phase microextraction: Application of medium‐chain carboxylic acid as extraction phase
Author(s) -
Ebrahimpour Behnam,
Yamini Yadollah,
Esrafili Ali
Publication year - 2013
Publication title -
journal of separation science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.72
H-Index - 102
eISSN - 1615-9314
pISSN - 1615-9306
DOI - 10.1002/jssc.201201022
Subject(s) - extraction (chemistry) , chromatography , ionic strength , tap water , chemistry , phase (matter) , detection limit , reagent , high performance liquid chromatography , analytical chemistry (journal) , seawater , solvent , ionic liquid , sample preparation , aqueous solution , biochemistry , oceanography , organic chemistry , environmental engineering , engineering , geology , catalysis
In this research, a novel homogeneous liquid‐phase microextraction method was successfully developed based on applying octanoic acid as low‐density extraction solvent. The method was applied for extraction and determination of chlorophenols ( CP s) as model compounds. Twelve milliliter of the sample solution was poured into a home‐designed glass vial. Sixty microliter of octanoic acid was solved in water sample by adjusting pH and ionic strength. By rapid addition of 75 μL of concentrated HC l (6 M), a cloudy solution was obtained. Phase separation occurred at 5000 rpm for 5 min. After that, 20 μL of the collected phase (approximately 26 μL) was injected into the HPLC‐UV instrument for analysis. The effect of some parameters such as the volume of concentrated HC l (phase separation reagent), ionic strength, extraction time, centrifugation time, and the volume of extracting phase on the extraction efficiency of the CP s were investigated and optimized. The preconcentration factors in a range of 159–218 were obtained under the optimal conditions. The linear range, detection limits ( S / N = 3), and precision ( n = 3) were 1– 200, 0.3–0.5 μg/L, and 4.6–5.1%, respectively. Tap water, seawater, and river water samples were successfully analyzed for the existence of CP s using the proposed method and satisfactory results were obtained.